Designing microarray oligonucleotide probes
نویسندگان
چکیده
منابع مشابه
Tutorial section: Designing microarray oligonucleotide probes
INTRODUCTION DNA microarrays are one of a few technologies that enable the monitoring of thousands of gene expression levels in parallel. In general, microarrays comprise a solid planar substrate on which an ordered array of probes has been deposited. Each probe represents a gene or transcript of interest and there are essentially three main types of probe: polymerase chain reaction (PCR) produ...
متن کاملTutorial section Designing microarray oligonucleotide probes
INTRODUCTION DNA microarrays are one of a few technologies that enable the monitoring of thousands of gene expression levels in parallel. In general, microarrays comprise a solid planar substrate on which an ordered array of probes has been deposited. Each probe represents a gene or transcript of interest and there are essentially three main types of probe: polymerase chain reaction (PCR) produ...
متن کاملMicroarray oligonucleotide probes.
Oligonucleotide probes are increasingly the method of choice for many modern DNA microarray applications. They provide higher target specificity, probe selection gives improved experimental control of hybridization properties, and targeting of specific gene subsequences allows better discrimination of highly similar targets such as splice variants or gene families. Only recently has there been ...
متن کاملCooperativity of paired oligonucleotide probes for microarray hybridization assays.
Synthetic DNA probes attached to microarrays usually range in length from 25 to 70 nucleotides. There is a compromise between short probes with lower sensitivity, which can be accurately synthesized in higher yields, and long probes with greater sensitivity but lower synthesis yields. Described here are microarrays printed with spots containing a mixture of two short probes, each designed to hy...
متن کاملDesigning Specific Oligonucleotide Probes for the Entire S. cerevisiae Transcriptome
Probe specificity plays a central role in designing accurate microarray hybridization assays. Current literature on specific probe design studies algorithmic approaches and their relationship with hybridization thermodynamics. In this work we address probe specificity properties under a stochastic model assumption and compare the results to actual behavior in genomic data. We develop efficient ...
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ژورنال
عنوان ژورنال: Briefings in Bioinformatics
سال: 2003
ISSN: 1467-5463,1477-4054
DOI: 10.1093/bib/4.4.361